Quantification of Protein Sulfenic Acid Modifications Using Isotope-Coded Dimedone and Iododimedone
نویسندگان
چکیده
منابع مشابه
Quantification of protein sulfenic acid modifications using isotope-coded dimedone and iododimedone.
Since its discovery almost 40 years ago, S-hydroxylation ( SOH) of cysteine thiol side chains at active and allosteric sites within proteins has emerged as a central post-translational modification. At present, more than 200 transcription factors, signaling proteins, metabolic enzymes, proteostasis regulators, and cytoskeletal components that undergo sulfenic acid modification have been identif...
متن کاملMeasurement of protein sulfenic acid content.
Protein sulfenic acids are reactive, reversibly oxidized cysteinyl residues with roles in redox catalysis and regulation. Detection and quantification of these species in proteins is accomplished through chemical modification by reagents such as 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (NBD chloride), 2-nitro-5-thiobenzoate (TNB), dimedone, or derivatives of dimedone, followed by UV-visible spec...
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Background: Bradford protein assay is popular due to its ease of performance and relative sensitivity. Many researchers and laboratories in Iran use standard assay of Bradford by cuvette. No commercial kit was available for Bradford microplate assay in Iran. Meanwhile, imported Bradford commercial kits are very expensive and have a long delivery time in Iran. Till now no study or document on Br...
متن کاملUse of dimedone-based chemical probes for sulfenic acid detection methods to visualize and identify labeled proteins.
Reversible thiol modification is a major component of the modulation of cell-signaling pathways by reactive oxygen species. Hydrogen peroxide, peroxynitrite, or lipid hydroperoxides are all able to oxidize cysteines to form cysteine sulfenic acids; this reactive intermediate can be directly reduced to thiol by cellular reductants such as thioredoxin or further participate in disulfide bond form...
متن کاملUse of dimedone-based chemical probes for sulfenic acid detection evaluation of conditions affecting probe incorporation into redox-sensitive proteins.
Sulfenic acids, formed as transient intermediates during the reaction of cysteine residues with peroxides, play significant roles in enzyme catalysis and regulation, and are also involved in the redox regulation of transcription factors and other signaling proteins. Therefore, interest in the identification of protein sulfenic acids has grown substantially in the past few years. Dimedone, which...
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ژورنال
عنوان ژورنال: Angewandte Chemie International Edition
سال: 2011
ISSN: 1433-7851
DOI: 10.1002/anie.201007175